Faculty Advisor

Shell, Scarlet

Abstract

Mycobacterium tuberculosis is a pathogenic bacterium that uses various adaptive mechanisms to deal with stress. The purpose of this project is to investigate one such mechanism, DNA methylation. To do this, we knocked down the expression of the methyltransferase MamA in Mycobacterium smegmatis. MamA deficient cells demonstrated growth cessation, elongation of the cell body, reduced distribution of DNA, and abnormal septation within cells. These results suggest that MamA could play a role in DNA replication and/or division.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2019

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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