Scarlata, Suzanne Frances
Phospholipase Cb1 (PLCb1), a component of the mammalian G-protein signaling pathway, moderates binding between tau, associated with neurodegeneration, and cyclin- dependent kinase 18 (CDK18). Prior literature suggests that CDK18 phosphorylates tau causing it to aggregate. These aggregates comprise neurofibrillary tangles and contribute to the loss of neurite function that are a hallmark of Alzheimer’s Disease. We previously found that PLCb1 binds CDK18 to inhibit its activity. To investigate PLCb1’s role in tau aggregation, fluorescence lifetime imaging microscopy (FLIM) and number and brightness (N&B) quantification were used. It was hypothesized that interfering with PLCb1- CDK18 binding would promote CDK18 tau phosphorylation and subsequently, aggregation.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry