Faculty Advisor

Heilman, Destin

Abstract

Enkurin is a novel protein first detected in mammalian sperm, and is thought to contribute to the maintenance of Ca++ ion channels. It was first observed in the acrosome (head) of mammalian sperm cells, but further localization studies found that it is also located in the principle piece (middle section) of flagellum. The purpose of this study was to determine the role of flagellar Enkurin in motility using Chlamydomonas reinhardtii, a unicellular biflagellated alga which expresses a homolog of mammalian Enkurin, as a model organism. Artificial microRNA (amiRNA) was introduced via electroporation to Chlamydomonas organisms normally expressing Enkurin in an attempt to silence the Enkurin gene and knock down protein production.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2010

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

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