Faculty Advisor

Buckholt, Michael Allan

Faculty Advisor

Gibson, Daniel G

Abstract

Ependymin is a neurotrophic factor found during certain cellular events related to neuronal development, cellular regeneration, and memory consolidation. Since its discovery in goldfish in 1976, ependymin has been partially sequenced and shows notable conservation through many different species. This allowed for the development of an 8 aa antibody against ependymin which can be used to identify the protein's presence in various tissues. Using immunohistological techniques we have attempted to determine the location of ependymin in several stages of Limulus development. Our preliminary fluorescent staining of whole-mount crabs indicates a strong presence of ependymin throughout the central nervous system in the 1st, 3rd and 4th juvenile instar stages.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2009

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Your accessibility may vary due to other restrictions.

Share

COinS