Faculty Advisor

Camesano, Terri Anne

Abstract

Escherichia coli O157:H7 contamination remains a significant concern for the food industry, as illustrated by several recent E. coli outbreaks through ground beef, fresh spinach, frozen pizza, raw cookie dough and Taco Bell. The ability of the antimicrobial peptide Cecropin P1-cys (CP1-cys) and an isolated fragment to bind and kill E. coli was evaluated using the Quartz Crystal Microbalance with Dissipation. Both CP1-cys and the fragment bound more and killed more E. coli O157:H7 and K12 than the bare adsorption surface. The isolated fragment is about 40% cheaper than the whole CP1-cys, which is a step towards fast, selective and cost-efficient biosensor applications.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2010

Major

Chemical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemical Engineering

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