Faculty Advisor

Adams, David S.

Abstract

The purpose of this project was to determine whether neutrophil-related proteins, S100A8 and CXCL5 correlate with the levels of Mycobacterium tuberculosis in mouse models, and explore whether these proteins could be used as indicators of disease progression. Their concentrations in the lungs were calculated using sandwich ELISAs, and bacterial lung burdens were calculated from Colony Forming Units (CFUs) on agar plates. Final analyses showed no correlation between neutrophil chemokines and M. tuberculosis burden, and that mice had no weight loss throughout the experimental timeframe. Therefore, when there is a weight gain, lung concentrations of S100A8 and CXCL5 cannot be used as indicators of disease progression in TB.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2019

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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