Roberts, Louis Anthony
University of Massachusetts Medical School
Tuberculosis is a bacterial infection caused by Mycobacterium tuberculosis, commonly presents as an infection of the alveolar sacs and can be difficult to treat. Granulysin, a lytic enzyme employed by CD8 cells during infection, has been shown to degrade M. tuberculosis. This project utilizes cloning, transfection, flow cytometry, and qPCR to build lentivirus and gamma-retrovirus expressing three isoforms of granulysin. These viruses can be used to infect CD8 cells and express human granulysin to better study granulysin’s interaction with Mycobacterium tuberculosis. Gaining a more complete understanding of these interactions can help identify future targets to help develop a new vaccine against tuberculosis.
Worcester Polytechnic Institute
Biology and Biotechnology
Major Qualifying Project
All authors have granted to WPI a nonexclusive royalty-free license to distribute copies of the work, subject to other agreements. Copyright is held by the author or authors, with all rights reserved, unless otherwise noted.