Faculty Advisor

Roberts, Louis Anthony

Abstract

Tuberculosis is a bacterial infection caused by Mycobacterium tuberculosis, commonly presents as an infection of the alveolar sacs and can be difficult to treat. Granulysin, a lytic enzyme employed by CD8 cells during infection, has been shown to degrade M. tuberculosis. This project utilizes cloning, transfection, flow cytometry, and qPCR to build lentivirus and gamma-retrovirus expressing three isoforms of granulysin. These viruses can be used to infect CD8 cells and express human granulysin to better study granulysin’s interaction with Mycobacterium tuberculosis. Gaining a more complete understanding of these interactions can help identify future targets to help develop a new vaccine against tuberculosis.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2019

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Share

COinS