Ryder, Elizabeth F.
VBH-1 is a DEAD-box RNA helicase found to co-immunoprecipate with CSR-1, an argonaute required for RNA-induced epigenetic activation (RNAa). Because most argonautes are thought to require an RNA helicase to function, this CO-IP data suggests that VBH-1 may be the helicase recruited by CSR-1. To test this hypothesis, experiments were conducted to assess CSR-1 function in vbh-1 mutants. These experiments found that mutations in VBH-1 do not hinder CSR-1 localization, though do result in a reduction of brood size, as well as a reduction in protein expression of CSR-1 targets. Future experiments will be carried out to assess if mutations in VBH-1 affect CSR-1 targeting.
Worcester Polytechnic Institute
Biology and Biotechnology
Major Qualifying Project
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