Burdette, Shawn C
A zinc ternary photocaged complex has been designed to passively diffuse through a cell membrane. Photocaged complexes have previously been used to release metal ions in biological systems with temporal and spatial control. This study used 3 ionophores with the newly developed DPAdeCage-zinc complex to test the formation of an overall neutral complex. 1H NMR titrations showed evidence of ternary complex formation with 1 ionophore: pyrithione. DPAdeCage-zinc with the pyrithione ionophore produced increased fluorescence in Zinpyr-1 labeled human fibroblasts, and decreased fluorescence after the addition of TPEN, a non-fluorescent cell permeable complex with a high affinity for zinc. These results suggest that zinc metal ions were introduced to the cells by way of the photocaged complex.
Worcester Polytechnic Institute
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Chemistry and Biochemistry
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