Artemisinin is an anti-malarial produced by the plant Artemisia annua. In the Weathers lab, one high producing artemisinin chemotype, nS, and a low producing artemisinin chemotype, n15, are used to study how the artemisinin pathway is regulated in A. annua. Prior metabolite data of cross-grafted plants indicated that the rootstock of nS stimulated artemisinin production in n15 shoots, and n15 rootstocks inhibited artemisinin production in nS shoots. In this study, I measured transcripts of two transcription factors, AaWRKY1 and AaERF1, and one artemisinic enzyme, DBR2. Results showed that the transcription factor AaWRKY1 was significantly down regulated in the shoots of the 15/S grafts. Further experiments are needed to explain these results in context of the correlating metabolite data.
Worcester Polytechnic Institute
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