Müllerian Inhibiting Substance (MIS) works through the Bone Morphogenetic Protein pathway, which utilizes the Bone Morphogenetic Response Element(BRE), resulting in apoptosis of ovarian cancer stem cells. This MQP focused on the development of new analytical and developmental approaches for purification and detection of rhMIS. The rhMIS N-terminus is known to stabilize the rhMIS C-terminus. MIS Type II receptor positive OVCAR-8 cells were transfected with a BRE-Luciferase report plasmid to identify the effect of active rhMIS. OVCAR-8 cells proved to be unacceptable hosts to create a stable BRE-Luc assay. Contrary to published reports, the relative affinity of rhMIS N-terminus appears not high enough to be used as an affinity ligand for rhMIS C-terminus.
Worcester Polytechnic Institute
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Chemistry and Biochemistry
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