Ex-vivo Characterization of the Lipolytic Response of Mouse Adipose Tissue to Endocrine Stimuli
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open in viewerFasting improves insulin sensitivity by increasing cellular uptake of glucose which affects lipolysis. To better characterize the endocrine function of visceral white adipose tissue (WAT) an ex-vivo model using tissue explants from fasted and non-fasted DBA/2J mice was tested. Endocrine stimulators used were B-adrenergic (BA), isopreternol, and adenylyl cyclase (AC), forskoline, receptor stimulators. Lipolysis was measured by non-esterified fatty acid (NEFA) and glycerol concentrations in media. With isopreternol, fasted mice showed a more than 10- fold increase in NEFA secretion than non-fasted mice; BA pathway increased lipolysis significantly more than AC stimulation in mouse WAT. Together, these findings indicate that hormones play a major role in WAT lipolytic function.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-042513-124101
- Advisor
- Year
- 2013
- Sponsor
- Date created
- 2013-04-25
- Resource type
- Major
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