Faculty Advisor

Roberts, Louis Anthony

Faculty Advisor

Farny, Natalie

Abstract

Fragile X Syndrome is characterized by loss of function of FMRP, which causes autism spectrum disorders and mental retardation. Stress granules (SGs) are dynamic aggregations of stalled mRNA and protein formed during the cellular stress response to halt their translation and promote cell survival. FMRP reversibly halts translation in cells, and loss of function may result in upregulated translation in FXS-affected cells. Therefore, we hypothesized that FXS cells would form SGs at a faster rate, and/or at a lower level of environmental stressor, than wild type cells. We used fluorescent microscopy to quantify SG formation in wild type and FXS-affected human B lymphocytes. Our results suggest that loss of FMRP does not contribute to higher stress sensitivity in FXS-affected cells.

Publisher

Worcester Polytechnic Institute

Date Accepted

2020-05-16

Major

Biology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Advisor Department

Biology

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