Faculty Advisor

Buckholt, Michael Allan

Faculty Advisor

Dominko, Tanja

Abstract

Following discoveries about the role that human basic fibroblast growth factor (bFGF or FGF-2) plays in cell reprogramming events, this project endeavored to establish a mammalian cell platform for further study of these proteins and their functions. FGF2 is encoded by a single mRNA , which utilizes the discrete 5' start sites resulting in production of five FGF2 protein isoforms. The five isoform sequences were originally cloned into separate pBluescript vectors. The sequences were then subcloned into a mammalian expression vector, and the protein was overexpressed in CHO-K1 cells where production and localization were verified by immunoblotting. We report production of two of the five protein isoforms as recombinant human proteins.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2011

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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