Rolle, Marsha W.
A potential application for tissue engineering is the development of functional, three-dimensional vascular constructs to aid in drug discovery. The goal of the project was to create a device that measures the contractile force of vascular ring constructs in response to chemical stimulation to model blood vessel function in vitro. A polydimethylsiloxane (PDMS) device consisting of a well and a center base with four extruding microposts was developed. Tissue rings were mounted on the posts and exposed to saline solution with or without potassium. Potassium-mediated smooth muscle contraction caused micropost deflection, which was measured with a stereomicroscope and used to calculate force. This presents a method to quantify the contraction response of tissue ring constructs.
Worcester Polytechnic Institute
Major Qualifying Project