Faculty Advisor

Heilman, Destin

Abstract

Gene regulation is an integral part of cellular function and key to understanding how genetic material is expressed. One of the regulatory pathways is hypothesized to be the interaction of sense and antisense transcripts that leads to the degradation of the dsRNA and consequently regulation. This model was investigated by direct transcript analysis by qRT-PCR and a RFP/GFP reporter that changes expression of fluorescent proteins in vivo. The analysis suggested a more biologically complex interaction. The development of this assay is under construction and is needed for the confirmation that antisense transcription is part of gene regulation. The data could suggest a case where antisense transcription plays a role in gene regulation adding to the complexity of the RNAi pathway.

Publisher

Worcester Polytechnic Institute

Date Accepted

May 2014

Major

Chemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

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