UMASS Medical Center
Gene regulation is an integral part of cellular function and key to understanding how genetic material is expressed. One of the regulatory pathways is hypothesized to be the interaction of sense and antisense transcripts that leads to the degradation of the dsRNA and consequently regulation. This model was investigated by direct transcript analysis by qRT-PCR and a RFP/GFP reporter that changes expression of fluorescent proteins in vivo. The analysis suggested a more biologically complex interaction. The development of this assay is under construction and is needed for the confirmation that antisense transcription is part of gene regulation. The data could suggest a case where antisense transcription plays a role in gene regulation adding to the complexity of the RNAi pathway.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry