Genetic engineering is currently dominated by CRISPR/Cas9 technology, promising precise multipurpose genome editing. The 2016 WPI iGEM team investigated the potential of adapting this technology to direct single-base editing of mRNA by linking deactivated Cas9 to the C-to-U RNA editor enzyme APOBEC1. We pursued the re-cloning and characterization of the dCas9/APOBEC fusion to identify and eliminate problems related to expression and toxicity. Ultimately, this adapted CRISPR/Cas9 system could provide an advantageous method of editing, particularly for therapeutics.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Baker, Andrew Robert

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042617-104047

Advisor

• Buckholt, Michael Allan
• Farny, Natalie

Year

• 2017

Date created

• 2017-04-26

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

Last modified

• 2021-01-29