Faculty Advisor

Buckholt, Michael Allan

Faculty Advisor

Farny, Natalie

Abstract

Genetic engineering is currently dominated by CRISPR/Cas9 technology, promising precise multipurpose genome editing. The 2016 WPI iGEM team investigated the potential of adapting this technology to direct single-base editing of mRNA by linking deactivated Cas9 to the C-to-U RNA editor enzyme APOBEC1. We pursued the re-cloning and characterization of the dCas9/APOBEC fusion to identify and eliminate problems related to expression and toxicity. Ultimately, this adapted CRISPR/Cas9 system could provide an advantageous method of editing, particularly for therapeutics.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2017

Major

Biology and Biotechnology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Advisor Department

BIO

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