UMMC / University of Massachusetts Medical Center
Ernesto Soto, Ph.D
Glucan particles (GPs) are hollow, porous 2-4 micron microspheres derived from the cell walls of Bakers yeast. The glucan content on the surface of the particles allows for receptor mediated cell uptake by cells with beta-glucan receptors. GPs have been used for the delivery of macromolecules encapsulated inside the hollow GPs via layer-by-layer synthesis. In this project, the outer surface of GPs was chemically derivatized to introduce different charged functional groups. The modified GPs were evaluated for charged nanoparticle (aminated latex and carboxylated polystyrene) and soluble payload (i.e. siRNA, doxorubicin) surface binding and for efficient GP-mediated payload delivery to a model murine GP phagocytic cell line (NIH 3T3-D1).
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry