Faculty Advisor

Adams, David S.

Center

UMMC / University of Massachusetts Medical Center

Abstract

Glucose Regulated Protein-78 (GRP78) is an ER stress chaperone whose decreased expression is associated with Type II diabetes. The goal of this project was to create a knockout mouse for GRP78 in adipocytes to allow future studies on the effects of GRP78 absence in that tissue. Mice hemizygous for Cre-recombinase under the control of the adipocyte-specific and tamoxifen-inducible promoter adiponectin (Ad-Cre) were bred with mice heterozygous for floxed GRP78 to produce a subset of pups of the desired genotype (homozygous floxed GRP78, hemizygous for Ad-Cre). Pups were screened by PCR genotyping tail snip DNA for Ad-Cre and GRP78. Two of the F1 male pups were of the exact desired genotype.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biology and Biotechnology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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