Histone deposition proteins CAF-1 and Asf-1 impact multiple aspects of chromosome formation and are essential in yeast to prevent DNA damage and avoid chromosomal rearrangement. Using fractionated yeast cell extracts, this MQP attempted to identify stimulatory factors which are necessary and sufficient for histone deposition by CAF-1 and/or Asf1. Identification of these cofactors, would provide insight into the reaction pathway and mechanism of histone deposition. Results were inconclusive, but improvements were proposed for the future success of this project.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Hall, Megan Joy

Contributors

• Smith, Corey

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042607-140705

Advisor

• Rulfs, Jill

Year

• 2007

Date created

• 2007-04-26

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright