Faculty Advisor

Adams, David S.

Abstract

Histone Nuclear Factor-P (HiNF-P) is the final link in a CyclinE/CDK2, p220NPAT pathway to cell cycle-dependent transcription of histone H4. In this project, mutations were introduced to HiNF-P to study the biological effects of the P-Specific Conserved Region (PSCR), a highly conserved region whose specific biological activity is unknown. Assays were carried out to quantify expression (Western), subcellular localization (immunofluorescence), DNA binding (EMSA), and H4 promoter activity (luciferase). The data strongly suggest that the first twenty amino acids of the PSCR are essential for DNA binding.

Publisher

Worcester Polytechnic Institute

Date Accepted

December 2006

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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