Faculty Advisor

Adams, David S.

Center

University of Massachusetts Medical Center

Abstract

Huntington's disease (HD) is one of the most destructive progressive neurodegenerative disorders, and is caused by a genetic mutation in the HTT gene encoding Huntingtin. To assist Neil Aronin's ongoing HD research at UMass Medical Center, we developed a more efficient protocol for mouse cortical dissection and plating. In addition, we optimized a procedure for transducing cortical cells with lentiviruses containing various siRNAs and GFP, and assayed the percent knockdown by qRT-PCR. In the future, these procedures will help assess the role of previously identified protein partners of mutant Huntingtin that contribute to HD pathogenesis.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2011

Major

Biology and Biotechnology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Project Center

University of Massachusetts Medical Center

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