Faculty Advisor

Prusty Rao, Reeta

Abstract

Identification of flagellar motility mutants can directly impact cilia-related disease research as flagellar proteins are highly conserved between the green alga Chlamydomonas reinhardtii and humans. Forward and reverse genetic analysis approaches would be more efficient with an available mutant collection and identified sites of gene disruption. This project showed how inserting a DNA fragment into the C. reinhardtii genome resulted in cells with defective swimming. 35 mutants were generated, two of which had insertions in genes of interest. A flagellar protein ODA1 mutant showed decreased protein production, while a calcium ATPase mutant exposed to different calcium concentrations did not show any significant changes in swimming ability.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2011

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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