Faculty Advisor

Camesano, Terri Anne

Faculty Advisor

Dominko, Tanja

Abstract

A recombinant form of human antimicrobial polypeptide, LL-37 Cathelicidin, was designed and expressed in human cells with the goal for the peptide to incorporate into cell-derived extracellular matrix. This research project investigated cellular localization of the recombinant Cathelicidin, created stable H1299 cells producing the peptide, conducted initial testing to concentrate the peptide, tested the peptide’s antimicrobial potency against common dermal bacteria, and its attachment affinity to collagen and fibronectin surfaces. Cell-derived ECM with antimicrobial properties could be used for tissue engineering applications.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biology and Biotechnology

Major

Chemical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemical Engineering

Advisor Department

Biology and Biotechnology

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