Faculty Advisor

Adams, David S.

Abstract

In Drosophila melanogaster circadian rhythms can be entrained by light and temperature. Three essential proteins for the Drosophila circadian clock are PER, TIM, and the photoreceptor CRY. In cryb mutant flies that lack CRY, PER and TIM do not cycle under light/dark conditions. A hypothesis was made that PER and TIM would oscillate normally in a temperature cycle under constant darkness in cryb flies. Through immunoblotting it was found that PER and TIM oscillate in cryb flies similarly to that of wt, supporting the idea that CRY is only required for light responses. Behavioral tests were performed to see if adding a temperature cycle into a LD cycle would cause cryb fly activity to shift. Cryb flies showed greater sensitivity to temperature cycles than wt flies under these conditions.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2007

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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