Faculty Advisor

Adams, David S.

Center

University of Massachusetts Medical Center

Abstract

In Multiple Sclerosis patients, previous experiments have determined the structure, potential function, and regulation of the oxidative damage resistance protein nuclear receptor coactivator-7 alternate start (NCOA7-AS) through induction with Interferon-Beta (IFN-Beta). However, the subcellular localization of NCOA7-AS remains unknown. In this project, the localization will be determined by fusing GFP to NCOA7-AS, expressing the fusion protein in HT1080 and HeLa cells, and using fluorescence microscopy. Cells will also be treated with IFN-Beta to see if this alters NCOA7-AS localization. The GFP microscopy data indicate that NCOA7-AS localization is diffuse in cells with no clear subcellular localization, and that addition of IFN-Beta does not alter the localization.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2013

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Project Center

University of Massachusetts Medical Center

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