Title

A Method for Determining the Effect of Stiffness and Stretch on Cell Phenotype

Faculty Advisor

Billiar, Kristen

Faculty Advisor

Pins, George

Abstract

In order to elucidate the interrelated effects of mechanical stimuli on cell phenotype in vitro, we developed a method for culturing cells at various levels of substrate stiffness and equibiaxial stretch. Polydimethylsiloxane was polymerized on flexible silicone membranes at different ratios of elastomer base to crosslinker to modulate stiffness. The membranes were stretched using a vacuum-driven system. Image analysis verified the uniformity of the strain field applied to the cells. Microscopic analysis of stretched and static samples demonstrated cell viability.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2006

Major

Mechanical Engineering

Major

Biomedical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biomedical Engineering

Advisor Program

Biomedical Engineering

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