Student Work

Generation and Mitigation of Cell Debris in a Manufacturing Scale Disc Stack Centrifuge

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In partnership with Amgen, a study was conducted to understand centrifugation utilizing a disc stack centrifuge, specifically looking to identify and quantify the amount of extracellular matrix (ECM) fragments that are generated and understand why they are created. This stemmed from issues that occurred during large-scale manufacturing runs, such as filter fouling and low yield. Detailed testing in search of quantifying and identifying general free particles in the supernatant was completed. Additionally, data from production harvests was analyzed to determine possible correlations between centrifugation parameters to explain issues that occurred during processing.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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  • E-project-042418-212317
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  • 2018
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Date created
  • 2018-04-24
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Permanent link to this page: https://digital.wpi.edu/show/hx11xg978