Faculty Advisor

Heilman, Destin

Faculty Advisor

Kmiotek, Stephen J.

Abstract

In partnership with Amgen, a study was conducted to understand centrifugation utilizing a disc stack centrifuge, specifically looking to identify and quantify the amount of extracellular matrix (ECM) fragments that are generated and understand why they are created. This stemmed from issues that occurred during large-scale manufacturing runs, such as filter fouling and low yield. Detailed testing in search of quantifying and identifying general free particles in the supernatant was completed. Additionally, data from production harvests was analyzed to determine possible correlations between centrifugation parameters to explain issues that occurred during processing.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2018

Major

Interdisciplinary

Major

Chemical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

Advisor Department

Chemical Engineering

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